Application

This Anti-acetyl-Histone H3 (Lys9) Antibody, clone RM161 is validated for use in Western Blotting, Multiplexing for the detection of acetyl-Histone H3.

General description

Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.

Immunogen

A linear peptide corresponding to human Histone H3 acetylated at Lys9.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in untreated and sodium butyrate treated HeLa acid extract.

Western Blotting Analysis (WB): 0.25 µg/mL of this antibody detected acetyl-Histone H3 (Lys9) in sodium butyrate treated HeLa acid extract.

Specificity

This antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in Histone H3.

Target description

~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).